Zydus Lifesciences Pvt. Ltd Walk-in Interview .Zydus Lifesciences Pvt. Ltd Notification full details below.Interested and eligible candidates can attend Interview on scheduled time and venue..
Vacancy details:
- Department: Biotech – Cell and Gene Therapy (CGT) R&D and Manufacturing
- Experience: Freshers or candidates with 2-8 years of experience in Cell and Gene Therapy (CGT).
- Qualification: PhD, M.Tech, or M.Sc in Life Sciences, Biochemistry, Biotechnology, or Microbiology.
Important Details :
- Selection Process: The selection will be on the basis of Interview.
- Mode of Interview: Face to face
- Interview Rounds of Interview: HR
Walking Interview @ Pune
Location : Hotel Lemon Tree Premier, City Center, 15 & 15A, Connaught Rd, Modi Colony, Pune, Maharashtra 411001
Date & Time : 23rd Feb, 2025 (Sunday) & 09:00 am to 05:00 pm
Vacancy for Cell and Gene Therapy (CGT) R&D and manufacturing:
Qualifications/Experience PhD, M.Tech, or M.Sc in Life Sciences, Biochemistry, Biotechnology, or Microbiology. Freshers or candidates with 2-8 years of experience in Cell and Gene Therapy (CGT).
Lentiviral vector mfg process development
- Conducting upstream and downstream processes for lentivirus production.
- Preparing media and reagents.
- Generation of cell banks, reviving and maintenance of various mammalian cell lines.
- Performing transient and stable transfections for lentivirus generation.
- Optimizing processes to achieve high titers.
- Scaling up production in cell factories or bioreactors.
- Purifying and concentrating lentivirus using tangential flow/diafiltration and chromatography methods.
- Recording data and preparing regulatory documents.
CAR-T (Chimeric Antigen Receptor T cell) Manufacturing:
- Executing CAR-T cell production at lab scale and/or with automated/semi-automated systems.
- Preparing media and reagents.
- Handling human blood and cells.
- Isolating immune cells (PBMCs/T cells) from blood.
- Transducing cells with lentivirus.
- Expanding cells, formulating them, and cryopreserving.
- Reviving and maintaining cells.
- Recording data and preparing regulatory documents.
Quality Control:
- Performing in-process quality control (IPBiotech – Cell and Gene Therapy (CGT) R&D and Manufacturing) and release tests for CAR-T and lentivirus
- Preparing media and reagents.
- Conducting FACS-based analyses for cell characterization.
- Performing molecular biology assays (RT-PCR/qPCR, agarose gel electrophoresis, DNA sequencing) for characterizing lentivirus and CAR-T cells.
- Conducting cell-based assays for potency testing.
- Performing immunoassays for cytokine profiling.
- Validation/qualification of methods
- Recording data and preparing regulatory documents.
